Tag

Part:BBa_K237008

Designed by: Marc T. K. Nielsen   Group: iGEM09_SDU-Denmark   (2009-10-21)

OmpA leader sequence - Targeting to periplasm, cleaved during transport

Usage and Biology

We chose this localisation signal for use with our project BBa_K237003. Sadly we didn't finish this part, so we can't verify that it works, however numerous applications of it has been described in the litterature (see reference), so it is very fair to expect it to.

References

Recombinant protein secretion in Escherichia coli. F J M Mergulhão, D K Summers, and G A Monteiro Biotechnology advances 23 (3), 177-202 (May 2005) info:pmid/15763404 | info:doi/10.1016/j.biotechadv.2004.11.003

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None